Process Monitoring Protocols
Process Monitoring Protocols for Enhanced Anarobic Biormediation
Biodegradation of organic recalcitrant compounds stimulated by substrate addition creates measurable changes in the chemistry of groundwater in the treated area. By measuring these changes, it is possible to document and quantitatively evaluate the effect of adding EOS® to the subsurface to enhance anaerobic biodegradation at a site. Guidance on evaluating these protocols can be found in various publications on MNA and enhanced bioremediation including AFCEE et al. (2004), USEPA (1998), National Academy of Sciences (2000), ITRC (1999), and Morse et al. (1998). To assist in implementing an EOS® project, the Table below provides an outline of the parameters and methods that other practitioners have used to monitor the progress of an EOS®-based project.
Analysis Method / Reference Frequency of Analysis pH Field probe with direct reading meter calibrated in the field according to the supplier's specifications (field) Each sampling round Temperature Field probe with direct reading meter (field) Each sampling round Oxidation-Reduction Potential (ORP) A2580B Measurements made with field meter and ion-specific electrodes (field) Each sampling round Dissolved oxygen Dissolved oxygen meter calibrated in the field according to the supplier's specifications (field) Each sampling round Conductivity E120.1/SW9050, direct reading meter (field) Each sampling round Major Cations USEPA SW6010 (laboratory) Optional Aromatic and chlorinated hydrocarbons USEPA SW8260B (laboratory) Each sampling round Nitrate -N IC method E300 (laboratory) Each sampling round Iron (II) (Fe2+) Colorimetric Hach Method # 8146 (field) Each sampling round Sulfate (SO42-) IC method E300 (laboratory) or by Hach method # 8051 (field) Each sampling round Methane, ethane, and ethene Kampbell et al., 1989 or USEPA SW3810 Modified (laboratory) Each sampling round Total or Dissolved Organic Carbon (TOC/DOC) USEPA SW9060 (laboratory) Each sampling round
References
AFCEE. 2004. Principless and Practices of Enhanced Anaerobic Bioremediation of Chlorinated Solvents, Air Force Center for Environmental Excellence, San Antonio, Texas.
Interstate Technology and Regulatory Council (ITRC) Work Group. 1999. Technical and Regulatory Requirements for Enhanced In Situ Bioremediation of Chlorinated Solvents in Groundwater. December. www.itrcweb.org.
Morse, J.J., B.C. Alleman, J.M. Gossett, S.H. Zinder, D.E. Fennell, G.W. Sewell, and C.M. Vogel. 1998. Draft Technical Protocol: A Treatability Test for Evaluating the Potential Applicability of the Reductive Anaerobic Biological In Situ Treatment Technology (RABITT) to Remediate Chloroethenes. Prepared for ESTCP. February 23, 1998.
National Academy of Sciences. 2000. Natural Attenuation for Groundwater Remediation. Prepublication copy. www.nap.edu.
USEPA. 1998. Technical Protocol for Evaluating Natural Attenuation of Chlorinated Solvents in Groundwater. Cincinnati, OH: National Risk Management Research Laboratory, Office of Research and Development, USEPA. EPA/600/R-98/128.